Endocrine Abstracts

11BHSD1 is a NADP-dependant, bi-directional enzyme which inter-converts the glucocorticoid cortisol with its inactive metabolite cortisone. Follicular fluid (FF) from large antral ovarian follicles contains hydrophilic and hydrophobic compounds which stimulate and inhibit 11BHSD1 enzyme activities, respectively. The objective of this study was to assess whether levels of these enzyme modulators change in FF during development of porcine follicles. Pooled samples of FF, aspirat...

In the ovary, glucocorticoids are metabolised by 11βHSD enzymes. The bi-directional 11βHSD1 enzyme usually acts as an NADPH-dependent 11-ketoreductase to regenerate cortisol (F) from circulating cortisone (E), while 11βHSD 2 inactivates F using NAD+ as cofactor. Having isolated endogenous inhibitors of 11βHSD1 from porcine follicular fluid (pFF) and ovarian cysts, the aims of this study were to establish whether 11βHSD enzymes interconvert F...

The enzymatic inactivation of cortisol is catalysed by isoforms of 11βHSD. 11βHSD1 is a bi-directional, NADP(H)-dependent enzyme whereas11βHSD2 is an NAD+-dependent dehydrogenase that inactivates cortisol. In the male rat, 11βHSD1 is highly expressed in the testis, epididymis, vas deferens and vesicular glands. Although 11βHSD activities have recently been reported in boar testicular tissue, cortisol metabolism in the boar reproductive tract...

In the ovary, 11βHSD enzymes inter-convert cortisol (F) with its inert metabolite, cortisone (E). Porcine follicular fluid from antral follicles and spontaneous ovarian cysts contains hydrophobic compounds that inhibit NADP(H)-dependent cortisol metabolism by 11βHSD1. The aim of this study was to measure intra-follicular F:E ratios in porcine antral follicles and ovarian cysts, and determine whether F:E ratios correlate with the inhibition of 11βHSD1 by porcine ...